원문정보
초록
영어
The 10 species of marine green algae was collected from Jejudo(Island) in Korea. Methanolic and aqueous extracts were prepared and screened for inhibition activities against tumor cell growth. Of the tested samples, the sea staghorn (Codium fragile) aqueous extract (CFAE) showed the highest activity on CT-26 cell growth. Therefore, CFAE was selected for further
experiments and the possibility to induce apoptosis by the CFAE was investigated. Flow cytometric analysis revealed that it dosedependently increased apoptotic cells with hypodiploid DNA contents in CT-26 cell line. These results indicated that CFAE can suppress the growth of CT-26 cells through apoptosis. The CFAE decreased the protein expression of anti-apoptotic Bcl-xL and led to the activation of caspase-3 and -7. A crude polysaccharide was separated from CFAE and it mainly constituted with 61.2% galactose and 30.5% arabinose as analyzed by high performance liquid chromatography (HPLC).
목차
Introduction
Materials and Methods
Materials
Preparation of aqueous and methanolic extracts frommarine green algae
Cell culture
Cell growth inhibitory assay
Nuclear staining with Hoechst 33342
Flow cytometry analysis
Western blot analysis
Crude polysaccharide separation
Statistical analysis
Results and Discussion
Inhibitory effect of marine green algae extracts againstthe growth of tumor cells
Cytotoxic effect of CFAE in normal cells
Induction effect of apoptosis in CT-26 cells by CFAE
Effects of CFAE on apoptosis-related protein levels ofCT-26 cells
Inhibitory effect of CPS from CFAE against the growthof CT-26 cells
References