원문정보
초록
영어
The object of this study was to investigate the immune-enhancing effects of Chlorella vulgaris (CV) on a deteriorated immune function by a protein-energy malnutrition (PEM) diet. Unicellular algae, CV were used as a biological response modifier. Male C57BL/6J mice were fed for 15 days with standard diet or a PEM diet, which is associated with decreased host immune defense. After 8 days, mice in the PEM diet group were orally administered by 0.05, 0.1, and 0.15 g/kg body weight of CV or distilled water. Nutritional parameters, and interferon (IFN)-γ levels were significantly increased in the blood serum of the CV (0.15 g/kg)-treated group (29.6±2.8 pg/mL) compared to the non-treated PEM group (4.1±0.4 pg/mL, p<0.05). In addition, cell proliferation and production of cytokines were investigated via a CV (0.01, 0.1, and 1 mg/mL)
treatment using a human T cell line MOLT-4 cell. The CV treatment (1 mg/mL) significantly increased the production of both IFN-γ and interleukin (IL)-2 (51.3±3.4 and 285.9±18.8 pg/mL, respectively) compared to the control (51.3±3.4 and 442.6±14.3 pg/mL, respectively), but did not affect the production of IL-4. These results suggest that CV may be useful in improving the immune function.
목차
Introduction
Materials and Methods
Animals
Treatment of CV
Preparation and ingredient analysis of blood serum
Counting mononuclear cell suspensions from spleen,superficial and mesenteric lymph nodes
MOLT-4 cell cultures
MTT assay
Cytokine assay
Statistical analysis
Results and Discussion
Effect of CV on blood nutritional parameters
Effect of CV on mononuclear cell numbers in thespleen and the superficial and mesenteric lymph nodes
Effect of CV on the production of IFN-γ in blood serum
Effect of CV on human T cell line MOLT-4 cellproliferation
Effect of CV on the production of IFN-γ, IL-2, and IL-4 in human T cell line MOLT-4 cells
References