원문정보
초록
영어
Covalent cross-links between a number of proteins and peptides explain why transglutaminase may be widely used by food processing industries. The objective of this work was optimization of the fermentation process to produce transglutaminase from a new microbial source, the Streptomyces sp. P20. The strategy adopted to modify the usual literature media was: (1) fractional factorial design (FFD) to elucidate the key medium ingredients, (2) central composite design (CCD) to optimise the concentration of the key components. Optimization of the medium resulted in not only an 86% increase in microbial transglutaminase activity as compared to the media cited in the literature, but also a reduction in the production cost. Optimal fermentation conditions - namely temperature and agitation rate - were also studied, using CCD methodology. Usual conditions of 30°C and 100 rpm were within the optimal area. All other parameters for enzyme production were experimentally proven to be optimum fermentation conditions.
목차
Introduction
Materials and Methods
Materials
Selective isolation and preservation of actinomycetes
Microorganisms selection for MTGase production
Preliminary tests Production of MTGase using a highviscosity medium:
Selection of the carbon and nitrogen sources for MTGaseproduction:
Optimization procedure for the production parametersand experimental design
Elucidation of the significant components using a FFD:
Optimization of key ingredient concentrations using aCCD:
Temperature and agitation optimization procedure andexperimental design:
Influence of inoculum size
Kinetic study
Results and Discussion
Microorganisms selection for MTGase production
Preliminary tests Production of MTGase using a highviscosity medium:
Selection of the carbon and nitrogen sources for MTGaseproduction:
Elucidation of the significant components using a FFD:
Optimization of key ingredient concentrations using aCCD:
Temperature and agitation optimization procedure andexperimental design:
Influence of inoculum size and kinetic study
References