원문정보
초록
영어
The objective of this study was to evaluate three verocytotoxin-producing Escherichia coli (VTEC) detection kits to detect the presence of VT genes: Doupath Verocytotoxin (GLISA) developed by MERCK, ProsPect Shiga Toxin E. coil (STEC) Microplate Assay (ELISA) developed by Remel, and a polymerase chain reaction method. Our laboratory verified artificially inoculated samples. All three methods could detect very low numbers of VTEC, but VT-PCR had the best sensitivity for VTEC detection. From April through September 2005, 257 ground-beefs from supermakets and traditional markets were examined for the presence of VTEC by polymerase chain reaction immediately after purchase and total viable counts (TVC) were determined. VTEC was isolated from 30 of 257 ground-beefs. A variety of serogroups was found, including 10 stains belonging to the virulence type EHEC, but major serogroups such as O157, O26 and O111 were nor found.
목차
서론
재료 및 방법
재료 및 분석기기
신속 검출방법(RApid Screening Methods)의 평가
신속검출방법의 검출한계
분쇄육에서의 장출혈성 대장균 분포조사
결과 및 고찰
균주의 순수배양(pure culture)을 이용한 평가
표준균주를 인위적으로 오염시킨 식육검체를 이용한 평가
신속검출방법의 검출한계
분쇄육에서의 장출혈성 대장균 분포조사
결론
문헌