원문정보
초록
영어
A non-instrumental immunochromatographic (ICG) strip-test and direct competitive enzyme-linked immunosorbent assay (DC-ELISA) for aflatoxin B1 (AFB1) determination were developed and optimized. The detection limits of ICG strip-test and DC-ELISA were 0.5 and 0.004 ng/mL, respectively, and these methods possessed a cross-reaction to aflatoxins. The results of spiked samples by both methods were coincided with the amount spiked AFB1 and the comparative analyses of 172 real samples by 2 immunoassays and high performance liquid chromatography (HPLC) showed a good agreement. Especially, the ICG strip-test is easier to perform and quicker, but less sensitivity than DC-ELISA. Both methods could analyze a high sample throughput with short time, but the sample throughput of ICG strip-test was better. Therefore, the ICG strip-test can be used as a simple, easy, non-instrumental, and fast screening technique for AFB1 determination.
목차
Introduction
Materials and Methods
Materials and chemicals
Preparation of immunoreagents
DC-ELISA
ICG strip-test
Sample preparation
Analysis of AFB1 in artificially spiked and natural samples
Results and Discussion
Characterization of DC-ELISA
Characterization of ICG strip-test
Assessment of the matrix effect from grain and feed samples
Analytical comparison of DC-ELISA and ICG striptest
Monitoring of AFB1 in natural samples
References