원문정보
초록
영어
Cancer chemopreventive effects can be exerted through the induction of phase II detoxification enzymes and the inhibition of inflammatory responses. In this study, the cancer chemopreventive effects and anti-inflammatory responses of 30 seaweed extracts were examined. The extracts of Dictyota coriacea and Cutleria cylindrica exhibited the high chemoprevention index, having 4.36 and 4.66, respectively. They also activated antioxidant response element at 100㎍/mL by about 3-fold while did not activate xenobiotic response element. Seven seaweed extracts, Ishige okamurae, Desmarestia ligulata, Desmarestia viridis, Dictyopteris divaricata, D. coriacea, Sargassum horneri, and Sargassum yezoense, showed significant inhibition on nitric oxide (NO) and prostaglandin E2 (PGE2) production in a dose-dependant manner in 5-20㎍/mL. These seaweed extracts could be used as food materials for cancer chemoprevention. D. coriacea could contain potential chemopreventive agents not only that regulate genes via an ARE-dependent mechanism but also prevent the inflammation through inhibition of NO and PGE2 production.
목차
Introduction
Materials and Methods
Seaweed materials
Preparation of 30 seaweed extracts
Cell culture
Cell viability
Quinone reductase assay
Transient transfection and ARE/XRE activation assay using CAT-ELISA
Measurement of nitrite production using Griess reagent
Measurement of PGE₂production by enzyme-linked immunosorbent assay(ELISA)
Results and Discussion
Chemoprevention index (CI) of seaweed extracts in Hepalclc7 cells
Activation of ARE by 4 seaweed extracts in HepG2 cells
Inhibition of NO production in RAW264.7 cells
Inhibition of PGE₂production in RAW264.7 cells
References