원문정보
초록
영어
Thirteen commercial antimicrobial products were examined to assess the sporicidal activity against Bacillus cereus spores at room temperature, 60 and 85℃. Neither the antimicrobials showed detectable antimicrobial activity against the B. cereus spores nor induced spore germination after the treatment at 0.5 or 1.0%(w/v, v/v) commercial antimicrobial agents at room temperature for 0.5 to 4 hr. However, when the antimicrobials such as chitosan, lactic acid, fermented pollen, grapefruit extract were applied with heat at 85℃ for 30 min, more than 1 log CFU/mL spores were additionally inactivated compared to only heat treatment without antimicrobials. Imposition of 60℃ to B. cereus spores with the higher concentration of 5.0%(v/v) lactic acid or 2.5%(w/v) thiamine dilaurylsulfate for the longer time incubation of 24 hr resulted in 3 log CFU/mL spore inactivation. This work showed that low concentrations of commercial antimicrobials by themselves did not inactivate B. cereus spores. However, when physical processes such as heat were combined together, antimicrobials showed a synergistic effect against B. cereus spores.
목차
Introduction
Materials and Methods
Strains and B. cereus spore manufacture
Heat treatment and D-values
Spore inactivation by antimicrobials
Spores germination
Application of various concentrations of antivicrobials
Results and Discussion
Effect of commercial antimicrobials on inactivation of B. cereus spores at room temperature
Effect of commercial antimicrobials on germination of B. cereus spores
Time to kill assay
Combination effect of the increased concentration of antimicrobials and pasteurization
References