원문정보
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초록
영어
A multiplex polymerase chain reaction (PCR) method was developed to simultaneously detect 3 events of genetically modified (GM) maize. The event-specific primers were used to discriminate the following 3 events of GM maize (DAS-59122-7, TC6275, and MIR604) using multiplex PCR method. The zein gene was used as an endogenous maize reference gene in the multiplex PCR detection. The primer pair Zein-FIR producing a 99 bp amplicon was used to amplify the zein gene. The primer JI-Das-F1/R1 for DAS-59122-7, JI-TC6275-F3/R3 for TC6275, and JI-MIR F1/R1 for MIR604 yielded an amplicon of 130, 162, and 197 bp, respectively. The detection limit of multiplex PCR was 1% for DAS-59122-7, TC6275, and MIR604 for one reaction.
목차
Abstract
Introduction
Materials and Methods
Maize samples
DNA extraction
Oligonucleotide primers
Multiplex PCR conditions
Results and Discussion
Specificity of primer pairs for a multiplex PCR
References
Introduction
Materials and Methods
Maize samples
DNA extraction
Oligonucleotide primers
Multiplex PCR conditions
Results and Discussion
Specificity of primer pairs for a multiplex PCR
References
저자정보
참고문헌
자료제공 : 네이버학술정보