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논문검색

Impact of Sodium Copper Chlorophyllin on Mercury absorption Using an in Vitro digestion with Human Intestinal Cell Model

원문정보

Han-Joon Hwang, Soon-Mi Shim

피인용수 : 0(자료제공 : 네이버학술정보)

초록

영어

The effects of sodium copper chlorophyllin (SCC) on bioaccessibility and uptake of mercury from fish were investigated using an in vitro digestion coupled with a Caco-2 cell. Fish along with SCC was subjected to a simulated in vitro digestion, which simulates both the gastric and small intestinal phase in vivo. Mercury bioaccessibility, the amount of mercury released from fish to aqueous phase following a digestion, was measured. Various amounts of SCC (0.1-25 mg) significantly reduced mercury bioaccessibility in a dose dependent manner by 49-89% compared to the negative control (fish without SCC) (p<0.05). Mercury bioaccessibility in varying molar ratios of mercury to positive control, 2,3-dimercapto-1-propane sulfonate (DMPS) was between 24 and 52%. Mercury uptake by Caco-2 cells from test media containing aqueous phase following in vitro digestion was measured after 6 hr incubation at 37℃. Cellular mercury uptake with increasing amount of SCC ranged from 0.352 to 0.052㎍ mercury/mg protein, while those in DMPS treatment were between 0.14 and 0.27㎍ mercury/mg protein. Our study suggests that SCC can reduce mercury absorption following fish consumption and may be efficient as a synthetic chelating agent for long term chronic mercury exposure in fish eating populations.

목차

Abstract
 Introduction
 Materials and Methods
  Materials
  Test meals preparation
  In vitro digestion
  Uptake of mercury by Caco-2 intestinal cells
  Assessment of toxicity and cellular viability
  Protein assay
  Determination of mercury
  Statistical analysis
 Results and Discussion
  Mercury bioaccessility
  Mercury uptake by Caco-2 cell
  Effect of SCC on mercury bioaccessibility
  Effects of SCC on mercury uptake by Caco-2 cells 
 References

저자정보

  • Han-Joon Hwang Department of Food and Biotechnology, Korea University
  • Soon-Mi Shim Research Institute of Human Ecology, Seoul National University

참고문헌

자료제공 : 네이버학술정보
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