원문정보
초록
영어
After overproduction of a recombinant β-galactosidase of Bifidobacterium infantis in Pichia pastoris, a synthesis of galacto-oligosaccharides (GOS) from 36% lactose using the enzyme (170.74 U/mg) was investigated. The transgalactosylation ratio reached up to 25.2% with 83.1% conversion of initial lactose and the maximum yield of GOS was 40.6%. The GOS syrup was composed of a 13.43% galacto-oligosaccharides, 5.06% lactose, and 8.76% monosaccharides. The prebiotic effect of GOS on the growth of bifidobacteria and lactobacilli strains was investigated in vitro. The maximum growth rate of Bifidobacterium breve and Lactobacillus acidophillus in GOS syrup (5%, v/v) media were 0.49 and 0.96/hr that are higher than those in 1%(w/v) galactose and 1%(w/v) lactose containing media. However, there was no significant difference between the specific growth rates of L. acidophillus in 1%(w/v) glucose and 5%(v/v) GOS syrup. Our data showed that GOS definitely promoted the growth of B. breve ATCC 15700T and L. acidophilus ATCC 33323.
목차
Introduction
Materials and Methods
Strains and plasmid
Media and culture conditions
Preparation of the recombinant β-galactosidase
Enzyme activity and protein assay
Analysis of GOS by high performance liquid chromatography(HPLC)
GOS synthesis
Growth experiments
Results and Discussion
Growth and enzyme production
HPLC analysis
GOS synthesis
Effect of GOS on B. breve B 20S and L. acidophilus L-5
References