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논문검색

Identification and Characterization of Phytochemicals from Peanut(Arachis hypogaea L) Pods

초록

영어

Methanol extracts of peanut (Arachis hypogaea L.) pods were chromatographed, which yielded 3 phytochemicals 1-3 including 5,7-dihydroxychromone (1), eriodictyol (2), and 3',4',5,7-tetrahydroxyflavone (3). To confirm the presence of isolated phytochemicals, the pods extracts were performed by high performance liquid chromatography coupled with a photodiode array detector (HPLC-PDA) and a mass spectrometric detector (MSD) with electrospray ionization (ESI). Optimum extraction conditions for phytochemical contents using peanut germplasm were obtained by employing 90% MeOH for 12 hr at room temperature and phytochemicals 1-3 showed significant differences with concentrations of 407.56±23.35, 52.92±5.11, and 2,024.34±134.18㎍/g, respectively. Under this optimal conditions, the contents of phytochemicals 1-3 in peanut pods of 3 Korea cultivars including 'Jakwang', 'Daekwang', and 'Palkwang' exhibited phytochemical 3 was the highest range of 1,338.01±5,162.93㎍/g, followed by phytochemical 1 (509.13-1,382.10㎍/g), and phytochemical 2 (25.12-186.85㎍/g), respectively. Moreover, 'Jakwang' exhibited the highest contents of phytochemical (1:1,362.10±52.49 , 2:186.85±17.69 , and 3:5,162.93±148.64㎍/g , respectively), whereas the lowest contents was found in the 'Daekwang' (1:590.13±22.23 , 2:25.12±2.45 , and 3:1,338.01±62.17㎍/g, respectively). These results suggest that the methanol extracts of peanut pods may possess health related benefits to humans owing to various known biological activities of phytochemicals 1-3.

목차

Abstract
 Introduction
 Materials and Methods
  Plant material
  Reagents
  Instruments
  Sample preparation, HPLC, and LC/MS analysis conditions
  Calibration curves of 3 Phytochemicals
  Statistical analys
 Results and Discussion
  Identiflacation of isolated phytochemicals
  Profile of phytochemicals
  LC/MS by phytochemicals from peanut pods
  Optimization of extraction conditions
  Comparison of phytochemical contents from the pods of peanut cultivars
 References

저자정보

  • Jin Hwan Lee Yeongnam Agricultural Research Institute, National Institute of Crop Science, Rural Development Administration, Ministry of Environment, NAKDONG River Basin Environmental Office
  • In-Youl Baek Yeongnam Agricultural Research Institute, National Institute of Crop Science, Rural Development Administration
  • Tae Joung Ha Yeongnam Agricultural Research Institute, National Institute of Crop Science, Rural Development Administration
  • Myoung-Gun Choung Department of Herbal Medicine Resource, Kangwon National University
  • Jong-Min Ko Yeongnam Agricultural Research Institute, National Institute of Crop Science, Rural Development Administration
  • Sea-Kwan Oh Gneetics and Breeding Division, National Institute of Crop Science, Rural Development Administration
  • Hyun-Tae Kim Yeongnam Agricultural Research Institute, National Institute of Crop Science, Rural Development Administration
  • Hyung Won Ryu Division of Applied life Science(BK21 Program) Environment Biotechnonogy-National Core Research Center
  • Keum-Yong Park Yeongnam Agricultural Research Institute, National Institute of Crop Science, Rural Development Administration
  • Ki Hun Park Division of Applied life Science(BK21 Program) Environment Biotechnonogy-National Core Research Center

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