원문정보
초록
영어
Methanol extracts of peanut (Arachis hypogaea L.) pods were chromatographed, which yielded 3 phytochemicals 1-3 including 5,7-dihydroxychromone (1), eriodictyol (2), and 3',4',5,7-tetrahydroxyflavone (3). To confirm the presence of isolated phytochemicals, the pods extracts were performed by high performance liquid chromatography coupled with a photodiode array detector (HPLC-PDA) and a mass spectrometric detector (MSD) with electrospray ionization (ESI). Optimum extraction conditions for phytochemical contents using peanut germplasm were obtained by employing 90% MeOH for 12 hr at room temperature and phytochemicals 1-3 showed significant differences with concentrations of 407.56±23.35, 52.92±5.11, and 2,024.34±134.18㎍/g, respectively. Under this optimal conditions, the contents of phytochemicals 1-3 in peanut pods of 3 Korea cultivars including 'Jakwang', 'Daekwang', and 'Palkwang' exhibited phytochemical 3 was the highest range of 1,338.01±5,162.93㎍/g, followed by phytochemical 1 (509.13-1,382.10㎍/g), and phytochemical 2 (25.12-186.85㎍/g), respectively. Moreover, 'Jakwang' exhibited the highest contents of phytochemical (1:1,362.10±52.49 , 2:186.85±17.69 , and 3:5,162.93±148.64㎍/g , respectively), whereas the lowest contents was found in the 'Daekwang' (1:590.13±22.23 , 2:25.12±2.45 , and 3:1,338.01±62.17㎍/g, respectively). These results suggest that the methanol extracts of peanut pods may possess health related benefits to humans owing to various known biological activities of phytochemicals 1-3.
목차
Introduction
Materials and Methods
Plant material
Reagents
Instruments
Sample preparation, HPLC, and LC/MS analysis conditions
Calibration curves of 3 Phytochemicals
Statistical analys
Results and Discussion
Identiflacation of isolated phytochemicals
Profile of phytochemicals
LC/MS by phytochemicals from peanut pods
Optimization of extraction conditions
Comparison of phytochemical contents from the pods of peanut cultivars
References