원문정보
초록
영어
In human beings, it is known that there is a correlation between the occurrence of acne and the ability to suppress sebum. Sebosuppression may be related to the inhibition of sebocyte proliferation, differentiation, and lipogenesis in sebaceous glands. To investigate the skin sebosuppressive activity of green tea extract, the in vivo effects of its flavonoid compounds on the androgen-dependent stimulation of pigmented macules in hamsters and performed in vitro experiments with human primary sebocytes were examined. Our results imply a dual activity of skin sebosuppression by green tea flavonoids; some catechins including epigallocatechin-3-gallate (EGCG) and gallocatechin-3-gallate (GCG) may reduce the differentiation of sebocytes by inhibiting PPAR-γ1 mRNA expression, whereas some flavonol glycosides including kaempferol may inhibit lipogenesis in sebaceous glands by decreasing levels of the mature form of sterol-sensitive response elements binding protein-1c (SREBP-1c). Therefore, green tea is a potentially effective material for use in the development of health foods or cosmetics for skin sebosuppression.
목차
Introduction
Materials and Methods
REagents and sebocyte culture
Measurement of green tea extract content
Growth inhibition assay of hamster flank organ macules
Semi-quanitative RT-PCR
Western blot of mature SREBP-1 protein in sebocytes
Results and Discussion
Analysis of flavonoid compunds in green tea extract
Effect of flavonoid compounds on androgen-dependent stimulation of pigmented macules
Effect of flavonoid compounds on PPAR-γ1 mRNA expression in sebocytes
Effect of flavonids on the maturation of SREBP-1c protein in sebocytes
References