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Parasporin-4, A Novel Cancer Cell-killing Protein Produced by Bacillus thuringiensis

원문정보

Kuniyo Inouye, Shiro Okumura, Eiichi Mizuki

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목차

Abstract
 Introduction
 A New screening Method for δ-Endotoxins fo B. thuringiensis
 Formation of the biosensor simulated a brush border membrane
 Interation between δ-endotoxin and BBM by the SPR-based system
 Comparison the methods for interaction of Cry toxins with the receptors
 Identification and Characterizatio of Parasporin-7
 Indentification of two cytotoxic proteins of the B. thuringlensis A1470 strain
 Morphological changes in cultured cells by the recombinant parasporin-4 protein
 Cytotoxic activity of parasporin-4 against various mammalian cells
 An Efficient Preparation Method for Parasporin-4
 Solubility of the recombinant pro-parasporin-4 in various solutioins
 Cytotoxic activity of the recombinant parasporin-4 processed by pepsin or proteinase K
 Purification of parasporin-4 from inclusion bodies in E-coli by the treatment with pepsin
 Future Research
 References

저자정보

  • Kuniyo Inouye Graduate School of Agriculture, Kyoto University, Sakyo-ku
  • Shiro Okumura Fukuoka Industrial Technology Centre
  • Eiichi Mizuki Fukuoka Industrial Technology Centre

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