원문정보
초록
영어
Sphingolipid production was investigated through Ganoderma lucidum-submerged cultivation. Crude sphingolipid obtained from G. lucidum was purified by methanol precipitation, Dowex AG DW-X8 (H+ form) cation exchange chromatography, and preparative thin layer chromatography, Structure and functionalities of purified sphingolipid were elucidated including cutaneous hydration effect. Possibility of use as cosmetics material and new biomaterial was explored. Production was 0.4 g/L at 1% yield. Purified sphingolipid was identified as D-ribo-1,3,4-trihydroxy-2-aminoocta decan through UV/VIS, FT-IR, and 1H-NMR. Sphingolipids increased skinmate value for cutaneous hydration effect by 20% at 500 μg/mL and decreased skin roughness at 100 μg/mL. Results suggest shingolipids from G. lucidum are effective for cutaneous hydration and improvement of skin roughness.
한국어
Ganoderma lucidum 균사체의 액체 배양을 통하여 균체 내 sphingolipid를 분리 정제하여 그 구조를 규명하였으며, 피부보습을 비롯한 각종의 기능성을 탐색하여 화장품 소재 및 새로운 생물 산업소재의 이용가능성을 검토한 결과 배양한 Ganoderma lucidum로부터 일련의 과정을 통하여 crude sphingolipids를 얻고, methanol침전, Dowex AG 50W-X8(H2) cation exchange chromatography 및 preparative thin layer chromatography를 행하여 sphingolipids를 분리하였다. 생산량은 0.4g/L였으며, 수율은 1%였다. UV/VIS spectrum, FT-IR 및 1H-NMR 분석을 행하여 구조를 해석한 결과 phytosphingosine 유도체로 판명되었다. 이 phytosphingosine 유도체의 피부에 대한 보습효과는 500μg/mL 이상의 농도에서 약 20% 정도 skinmate 값이 증가하였으며, 100μ/mL 이상의 농도에서 거칠기 감소에 유의한 결과를 나타내었다. 이상의 결과로부터 Ganoderma lucidum이 생산하는 sphingolipids는 피부보습 및 거칠기 개선에 유의성이 확인되어 화장품 소재 및 새로운 생물 산업소재로서의 이용가능성이 높다고 할 수 있다.
저자정보
참고문헌
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