earticle

영어

As a study on the acid protease production by Rhizopus japonicus S-62, the culture conditions for the enzyme production and characteristics of the crude enzyme were investigated. The results are summarized as follows: 1. The optimum conditions for solid culture on wheat bran were 48 hrs of culture period and 100~120% addition of tap water. 2. Of the several media, wheat bran medium was the most excellent in the enzyme production. 3. The addition of sucrose, NH₄CL fructose and NaH₂PO₄on wheat bran, respectively, increased largely the enzyme production. 4. The optimum pH for the enzyme action was pH 2.4~2.6, the optimum temperature was about 40℃, and the stable pH range was 2.5~5.0, the enzyme was stable below 40℃ and was inactivated abruptly above 40℃.

한국어

Rhizopus japonicus S-62가 생산(生産)하는 acid protease에 관(關)한 연구(硏究)로서, 효소(酵素)의 생산조건(生産條件) 및 조효소(粗酵素)의 특성(特性)을 검토(檢討)하여 다음과 같은 결과(結果)를 얻었다. 1. 밀기울에 배양(培養)하는 경우 최적배양(最適培養) 시간(時間)은 48시간(時間) 첨수량(添水量)은 100~120%이었다. 2. 각종(各種) 배지중(培地中) 밀기울 배지(培地)가 가장 우수(優秀)하였다. 3. 밀기울에 sucrose, fructose, NH₄CL, NaH₂PO₄의 첨가(添加)는 protease생산(生産)을 크게 증가(增加)시켰다. 4.본효소(本酵素)의 작용최적(作用最適)pH는 2.4~2.6, Opt. temp.는 약(約) 40℃, 안정(安定) pH 범위(範圍)는 이고, 40℃이하(以下)에서는 안정(安定)하나 그 이상(以上)의 온도(溫度)에서는 급격(急激)하게 불활성화(不活性化)되었다.