earticle

영어

The initiation of immunoglobulin E (IgE)-mediated allergic reactions requires binding of IgE antibody to its high-affinity receptor. Human basophilic KU812F cells express FccRI on the cell surface and act as effector cells in the allergic response. In this study, we investigated the effects of Scutellaria radix extract on the expression of the FccRI in human KU812F cells. Flow cytometric analysis showed that S. radix extract treatment caused a concentration-dependent decrease in FccRI expression on the cell surface. Furthermore, the level of FccRI a,B, and r chain mRNA in KU812F cells was examined by RT-PCR. S. radix extract reduced total cellular and chain mRNA expression in a concentration-dependent manner. FccRI-mediated histamine release was reduced from 21.75±1.34 ng/106 cells in non-treated cells to 16.46±1.98 ng/106 cells in S. radix extract treated cells. These results suggested that S. radix extract has the potential to down-regulate of FcRI expression and to inactivate basophils.