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논문검색

Isolation and Characterization of Phytochemical Constituents from Soybean (Glycine max L. Merr.)

초록

영어

Four flavonoids 1-4 and one phytosterol 5 were isolated from methanol extract of Taekwangkong, one of the soybean cultivars, and the structures of these compounds were fully characterized by physical and spectral analysis. The content of compounds 1-4 as determined by C18 reversed phase HPLC (high-performance liquid chromatography) coupled with diode-array detector were 12.1, 624.6, 18.0, and 219.6 ug/g, respectively, and the total phenolic content of this cultivar was measured as 3.7 mg gallic acid equivalent per g dry material (GAB/g). Also, compound 1 showed strong radical scavenging activity in the 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) assay (IC50 = 47.6 uM), five-fold higher than seen in the 1,1-diphenyl-2-picrylliydrazyl (DPPH) assay. These results lead to the conclusion that soybean not only has many phytoestrogens but also has potent antioxidant activity.

목차

Abstract
 Introduction
 Materials and Methods
 Results and Discussion
 Acknowledgments
 References

저자정보

  • Lee, Jin-Hwan Yeongnam Agricultural Research Institute, National Institute of Crop Science, RDA
  • Baek, In-Youl Yeongnam Agricultural Research Institute, National Institute of Crop Science, RDA
  • Kang, Nam-Suk Yeongnam Agricultural Research Institute, National Institute of Crop Science, RDA
  • Ko, Jong-Min Yeongnam Agricultural Research Institute, National Institute of Crop Science, RDA
  • Han, Won-Young Yeongnam Agricultural Research Institute, National Institute of Crop Science, RDA
  • Kim, Hyun-Tae Yeongnam Agricultural Research Institute, National Institute of Crop Science, RDA
  • Oh, Ki-Won Yeongnam Agricultural Research Institute, National Institute of Crop Science, RDA
  • Suh, Duck-Yong Yeongnam Agricultural Research Institute, National Institute of Crop Science, RDA
  • Ha, Tae-Joung Yeongnam Agricultural Research Institute, National Institute of Crop Science, RDA
  • Park, Ki-Hun Division of Applied Life Science (BK21 program), Department of Agricultural Chemistry, Research Institute of Life Science, Gyeongsang National University

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