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Classification of Meat-Based Listeria monocytogenes Using Whole-Cell Protein Patterns and Serotyping Analysis

원문정보

Park, Si-Hong, Jung, Sang-Hoon, Kim, Hyun-Joong, Chung, Yun-Hee, Kim, Hae-Yeong

한국식품과학회 Food Science and Biotechnology Volume 15 Number 2 2006.04 pp.324-327
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초록

영어

The food-borne pathogen Listeria monocytogenes is commonly associated with meats and unpasteurized dairy products. To identify this pathogen in meats more efficiently than has been done in the past, we purchased meats from Korean markets and performed sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and serotyping analysis on Listeria organisms isolated from meat samples. Each Listeria species showed specific protein band patterns on SDS-PAGE. Whole-cell protein SDS-PAGE profiles indicated that the organisms isolated from meats sold in local Korean markets were L. monocytogenes with the serotypes 1/2a, 1/2b, 1/2c, and 4b. We suggest that it is possible to carry out molecular subtyping of L. monocytogenes using SDS-PAGE.

목차

Abstract
 Introduction
 Materials and Methods
 Results and Discussion
 Acknowledgments
 References

키워드

저자정보

  • Park, Si-Hong Department of Food Science and Biotechnology, Institute of Life Sciences and Resources, Kyung Hee University
  • Jung, Sang-Hoon Department of Food Science and Biotechnology, Institute of Life Sciences and Resources, Kyung Hee University
  • Kim, Hyun-Joong Department of Food Science and Biotechnology, Institute of Life Sciences and Resources, Kyung Hee University
  • Chung, Yun-Hee Test and Research Center, Korean Consumer Protection Board
  • Kim, Hae-Yeong Department of Food Science and Biotechnology, Institute of Life Sciences and Resources, Kyung Hee University

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