earticle

영어

In order to isolate hyaluronidase (HAase) inhibitor from Astragali radix (AR), dried roots were extracted with ethanol, prior to sequential fractionations with n-hexane, chloroform, ethyl acetate, n-butanol, and aqueous fractions. The n-butanol soluble fraction was found to exhibit the most pronounced inhibitory effect (68%) on HAase, and the active components were separated using various chromatographic methods, including column chromatography and preparative HPLC. The active component was isolated from the n-butanol soluble fraction of AR and was structurally identified as calycosin-7-O-B-D-glucopyranoside by LC-MS, IR, 1H NMR, and NMR analysis. The IC50 of calycosin-7-O-B-D-glucopyranoside's HAase activity was found to be 3.7 mg/mL.