earticle

영어

Optimal extraction conditions such as pressures, temperatures, and modifiers on glycyrrhizin extraction from licorice were investigated using supercritical CO2 (SC-CO2) at 3 mL/min flow rate. Morphology of licorice tissue, after glycyrrhizin extraction, was examined by SEM, and absolute density (g/cm3) measurement and glycyrrhizin content were determined by HPLC. Pure SC-CO2 had no effect on glycyrrhizin extraction, but recovery of glycyrrhizin (32.66±0.77%) was enhanced when water was used as modifier. The highest recovery was 97.22±2.17% when 70% (v/v) aqueous methanol was added to 15% (v/v) SC-CO2 at 50 MPa and 60℃. Under optimal extraction conditions, 30 MPa pressure and 60℃ heating temperature, glycyrrhizin recovery reached maximum (102.67±1.13%) within 60 min. Licorice tissue was severely damaged by excessive swelling, and absolute density of licorice residues was highest when aqueous methanol was used as a modifier.