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Purpose: This study was to analyze changes of motivation and health-promoting lifestyle in 3 months after medical examination, and to identify the influencing factors on the change of health-promoting lifestyle. Methods: The subjects of this study were 81 adults who took medical examination at a general hospital health clinic in Cheonan city. The instruments used in this study were the motivation scale and modified HPLP. For data collection, the first survey was conducted from March 16 to April 19, 2001, and the second survey was carried out by mail three months after the medical examination from June 16 to July 19, 2001. Results: 1. The age of the subjects ranged from 26 to 66 years, 71.6% were male, and the major group was office workers(43.2%). 2. The average score of motivation scale was significantly improved in three months to 520.7(SD=82.7). All sub-scales of motivation scale, self-efficacy(t=-4.204, p=.000), perceived benefits(t=-4.263, p=.000), perceived barriers (t=4.305, p=.000), and emotional salience (t=-6.169, p=.000) showed significant improvements in 3 months. 3. The average score of health-promoting lifestyle was significantly increased to 62.5 (SD=9.8) (t=-5.111, p=.000) after 3 months. Health responsibility(t=-6.098, p=.000), eating habit(t=-3.625, p=.001), exercise(t=-2.557, p=.012), and smoking habit(t=-2.157, p=.034) showed significant improvement. But stress management was not changed at the significant level in 3 months(t=-1.832, p=.071). 4. As the result of multiple regression analysis, it was found that perceived barriers, self-efficacy and monthly mean income had a significant influence on health-promoting lifestyle in 3 months after the medical examination. These variables explained 42.4% of variance in health- promoting lifestyle in 3 months after the medical examination. Conclusion: Periodic medical examination and guidance for healthy lifestyle was effective to change the motivation and to improve health promoting lifestyle.