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Purpose: This study aimed to empirically assess how the provident effect on shoulder muscle activity and stabilization varies depending on the range of physical application (upper body vs. whole body) of vibration to the quadruped position. Methods: Twenty healthy adult female Pilates students without shoulder pain or musculoskeletal disorders were recruited and divided into the upper-body (n=10) and whole-body (n=10) vibration groups. Pre- and post-exercise shoulder muscle activity (EMG) and shoulder stabilization tests (ROM, MMT, and YBT) were performed. Two-way analysis of variance (ANOVA) was performed to determine the differences in the effects of vibration application between groups. Results: Vibrations applied in the quadruped position exerted a statistically significant impact on shoulder muscle activity. The upper-body vibration group showed substantially greater improvement than the whole-body vibration group. Conclusion: This study confirmed that the magnitude of improvements due to different extents of musculature exposed to vibrations were distinct. Both the upper and whole-body vibration groups experienced significant improvement, with the upper-body vibration group experiencing greater shoulder muscle improvement than the whole-body vibration group.