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Purpose: This study aimed to investigate the effects of taping-assisted rhythmic stabilization exercises on shoulder muscle activity and function in patients with shoulder impingement syndrome. Methods: Twenty patients diagnosed with shoulder impingement syndrome were randomly assigned to experimental (n=10) or control (n=10) groups. The experimental group underwent rhythmic stabilization exercises combined with taping, while the control group underwent general physical therapy for 30 min per session, three times a week, for six weeks. Muscle activities of the upper trapezius (UT), lower trapezius (LT), and serratus anterior (SA) were measured using surface electromyography, and shoulder function was assessed using the Oxford Shoulder Score (OSS) before and after the intervention. Results: After the intervention, the experimental group demonstrated a significant increase in SA muscle activity and shoulder function compared with the control group (p<.05). Within-group analysis revealed significant improvements in SA activity and OSS only in the experimental group (p<.01), whereas no significant changes were observed in UT or LT muscle activity in either group. Conclusion: Taping-assisted rhythmic stabilization exercise was found to be an effective intervention for enhancing selective muscle activation and shoulder function in patients with shoulder impingement syndrome. This combined approach may serve as a valuable clinical rehabilitation strategy to improve shoulder stability and functional recovery.