earticle

영어

Bioassay and HPLC-UV guided fractionations of the crude extract of marine-derived Streptomyces sp. SNA-077 have led to the isolation of a red pigment, undecylprodigiosin. The chemical structure of undecylprodigiosin was revealed by the interpretation of NMR and mass spectroscopic (MS) data. Further, anti-melanogenic effects of undecylprodigiosin were investigated. First, the melanin contents of undecylprodigiosi-treated B16 cells were evaluated. Furthermore, undecylprodigiosin significantly inhibited the key enzymes involved in melanogenesis, including tyrosinase, tyrosinase related protein-1 (TYRP- 1), and dopachrome tautomerase (DCT). The mRNA and protein expression levels of Microphthalmiaassociated transcriptian factor (MiTF), a critical transcription factor for tyrosinase gene expression, were also suppressed by undecylprodigiosin treatment in B16 analyses. Collectively, our results suggest for the first time that undecylprodigiosin, a potent component isolated from an extract of marine Streptomyces sp. SNA-077, critically exerts the anti-melanogenic ability for melanin synthesis.