earticle

영어

The aim of this study was to investigate the therapeutic potency of Rehmanniae Radix extract (RRE) in in vitro and in vivo psoriasis models. HaCaT keratinocytes were stimulated with M5 (mixture of IL- 17A, IL-22, IL-1α, oncostatin M, and TNF-α) for development of a psoriatic keratinocyte model in vitro. The expression of Janus kinase 2/signal transducers and activators of transcription 3 (JAK2/STAT3) pathways were detected by western blot. In vivo experiment, the psoriasis-like skin inflammation mice model was established by 5% imiquimod (IMQ). RRE treatment significantly attenuated skin inflammation and improved skin integrity in imiquimod-treated mice by suppressing keratinocyte hyperproliferation, inhibiting the infiltration of immune cells, and downregulating the expression of psoriatic markers. Further, ADE treatment suppressed JAK2/STAT3 signaling in HaCaT cells. Overall, the application of RRE relieves psoriasis-like skin inflammation possibly by regulating the JAK2/STAT3 signaling pathways, making it an effective alternative for psoriasis therapy.