earticle

영어

This study evaluated the effects of Puerariae radixextract (PRE) on inflammatory dermatosis by in vitro and in vivo studies. And clarified the important role of the JAK/STAT3 signaling pathway in the treatment of psoriasis with PRE. In vitro, HaCaT cells were induced by M5 (mixture of IL-17A, IL-22, IL-1α, oncostatin M, and TNF-α) to establish an inflammatory injury model, and the effects of PRE on inflammation. The expression of NF-κB and JAK/STAT3 pathways were detected by western blot. Combined with an in vivo mice model of Imiquimod (IMQ) induced psoriasis-like skin inflammation, the most effective PRE for alleviating psoriasis was identified and its potential mechanism was investigated. PRE effectively downregulate inflammatory factors (IFN-γ, CCL20, IL-6 and TNF-α) protein levels. Western blot analysis indicated that PRE has anti-inflammatory effects by inhibiting the activation of NF-κB and JAK/STAT3 pathways. In vivo experiment, the psoriasis-like skin inflammation mice model was established by 5% IMQ and treated PRE for 7 days, the results showed that PRE intervention reduced the skin scale and significantly inhibited the secretion of inflammatory factors in skin lesions. Puerariae radixextracts were effective in improving skin inflammation and alleviated psoriasis through JAK/STAT3 pathway. The results provided experimental support for the potential use of Puerariae radixin functional food and skin care products.