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Bio and medical Information Technology (BIT)

Correlation between Hair Cuticle Damage and Color (Lightness) Alteration Following Repeated Non-Thermal Bleaching : A Comparative Study by Hydrogen Peroxide Concentration

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영어

Purpose: We aimed to investigate the relationship between hydrogen peroxide (H₂O₂) concentration, the number of non-thermal bleaching sessions, and their effects on hair lightness and cuticle damage. This study seeks to establish a scientific foundation for safer bleaching protocols, particularly for fragile or chemically treated hair. Methods We conducted repeated non-thermal bleaching using 3%, 6%, and 9% H₂O₂ on a total of 46 human hair tresses. Each concentration group included three sets of five tresses (total n=45), and one untreated virgin hair tress was used as a control. Hair lightness was measured using a standardized 20-level chart, and cuticle damage was assessed via FE-SEM (× 2,000). Damage types—lifting, fragmentation, and dissolution—were classified morphologically. Results We observed that as H₂O₂ concentration and bleaching frequency increased, hair lightness rose, but so did the severity and extent of cuticle damage. Notably, the 9% group reached Level 19 lightness by the third session but exhibited widespread cuticle dissolution. Conversely, the 3% group reached only Level 17 after five repetitions and showed milder damage. At similar lightness (Level 17), the 6% group showed more severe cuticle degradation than the 3% group. This reveals that concentration plays a more critical role than frequency in determining damage severity. Conclusion: We confirmed that equivalent levels of lightness can mask significantly different levels of cuticle integrity depending on the bleaching protocol. Relying solely on lightness as a damage indicator is insufficient. Our study highlights the importance of morphological analysis of the cuticle to assess structural damage and supports the use of low-concentration H₂O₂ with controlled repetition in professional settings. We propose further research using image quantification and diverse hair types to improve generalizability and reproducibility.

목차

Abstract
1. Introduction
2. Research Methods
2.1 Materials
2.2 Methods
3. Results
3.1 Comparative Analysis of Hair Color (Lightness) Change and Cuticle Damage According to Repeated Bleaching with 3% H₂O₂ Under Non-Thermal Conditions
3.2 Comparative Analysis of Hair Color (Lightness) Change and Cuticle Damage According to Repeated Bleaching with 6% H₂O₂ Under Non-Thermal Conditions
3.3 Comparative Analysis of Hair Color (Lightness) Change and Cuticle Damage According to Repeated Bleaching with 9% H₂O₂ Under Non-Thermal Conditions
3.4 Cumulative Changes in Hair Lightness and Morphological Cuticle Damage According to H₂O₂ Concentration and Bleaching Frequency
3.5 Statistical Analysis Results
4. Discussion
5. Conclusion
References

저자정보

  • Dae won Lee Master of Science, Dankook University Graduate School of Health and Welfare, Department of Clinical Medicine, Beauty and Anti-Aging Major
  • Young sam Yuk Professor, Dankook University Liberal Arts College Clinical Beauty Major

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