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논문검색

Effects of Oocyte Maturation Age and Activation Condition on the Development of Porcine Parthenogenetic Embryos

초록

영어

This study was conducted to investigate the effects of oocyte maturational age and activation condition on in vitro development of porcine parthenogenetic embryos (parthenotes). Porcine follicular oocytes were matured in vitro for 30 to 44 hr. Maturation rate was examined during in vitro maturation (IVM) every 2 hr interval. The cdc2 kinase activity was measured at 36 and 44 hr of IVM. Some oocytes were activated at 36 or 44 hr of IVM by three different conditions; 1) single electric stimulation (1.5 kV/cm for 30 sec; ES), 2) double electric stimulations (1.5 kV/cm for 30 sec, followed by 1.0 kV/cm for 50 sec after 1 hr; ES+ES) or 3) ES+ES followed by culture in 6-dimethlyaminopurine (6-DMAP) for 4 hr (ES+ES+D), and cultured for 6~7 days. Maturation rate was significantly increased as culture period was increased to 36 hr (66.9%, p<0.05), and then gradually increased to 87.1% at 44 hr of IVM. The cdc2 kinase activity was decreased (p<0.05) with culture period prolonged from 36 hr to 44 hr. Lower blastocyst formation rate (4.3%, p<0.05) were obtained by ES in 36 hr-matured oocytes compared to other treatments (16.5 and 20.5%) in the same age and the same treatment in 44 hr-matured oocytes (15.0%). High blastocyst formation rate (23.6%) was obtained by ES+ES+D in 44 hr-matured oocytes (p<0.05). These results demonstrate that porcine oocyte activation and in vitro development of parthenotes can be affected by interactions between oocyte maturational age and activation condition.

목차

ABSTRACT
 INTRIDUSTION
 MATERIALS AND METHODS
  In Vitro Maturation of Oocytes
  Evaluation of Nuclear Maturation
  MPF Kinase Assay
  Parthenogenetic Activation
  In Vitro Culture
  Statistical Analysis
 RESULTS
  Maturation Pattenrs of Porcine Oocytes
  cdc2 Kinase Activity
  Development of Parthenotes
 DISCUSSION
 REFERENCES

저자정보

  • Dae-Jin Kwon School of Veterinary Medicine
  • Joo-Hee Park School of Veterinary Medicine
  • Choon-Keun Park College of Animal Life Science, Kangwon National University
  • Boo-Keun Yang College of Animal Life Science, Kangwon National University
  • Hee-Tae Cheong School of Veterinary Medicine

참고문헌

자료제공 : 네이버학술정보
  • 1(2000) Effects of wortmannin on the kinetics of GVBD and the activities of the maturation-promoting factor and mitogen-activated protein kinase during bovine oocyte maturation in vitro,
  • 2(1999) Production of goats by somatic cell nuclear transfer,
  • 3(2000) Development of reconstituted pig embryos by nuclear transfer of cultured cumulus cells,
  • 4(2002) Effect of elevated Ca2+ concentration in fusion/activation medium on the fusion and development of porcine fetal fibroblast nuclear transfer embryos,
  • 5(1995) Beneficial effect of oocyte activation prior to and during nuclear transfer in cattle using in vitro matured oocytes 24 hr of age,
  • 6(1992) Intracellular Ca2+ response of rabbit oocytes to electrical stimulation,
  • 7(1995) Pronuclear formation and intracellular glutathione content of in vitro-matured porcine oocytes following in vitro fertilization and/or electrical activation,
  • 8(1991) Response of porcine oocytes to electrical and chemical activation during maturation in vitro,
  • 9(2001) Progression of nuclear maturation and p34cdc2 kinase activity in porcine oocytes during in vitro culture in different media,
  • 10(2000) Requirement of mitogen-activated protein kinase activation for the meiotic resumption of porcine oocytes,

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