원문정보
초록
영어
This study was conducted to investigate the effects of oocyte maturational age and activation condition on in vitro development of porcine parthenogenetic embryos (parthenotes). Porcine follicular oocytes were matured in vitro for 30 to 44 hr. Maturation rate was examined during in vitro maturation (IVM) every 2 hr interval. The cdc2 kinase activity was measured at 36 and 44 hr of IVM. Some oocytes were activated at 36 or 44 hr of IVM by three different conditions; 1) single electric stimulation (1.5 kV/cm for 30 sec; ES), 2) double electric stimulations (1.5 kV/cm for 30 sec, followed by 1.0 kV/cm for 50 sec after 1 hr; ES+ES) or 3) ES+ES followed by culture in 6-dimethlyaminopurine (6-DMAP) for 4 hr (ES+ES+D), and cultured for 6~7 days. Maturation rate was significantly increased as culture period was increased to 36 hr (66.9%, p<0.05), and then gradually increased to 87.1% at 44 hr of IVM. The cdc2 kinase activity was decreased (p<0.05) with culture period prolonged from 36 hr to 44 hr. Lower blastocyst formation rate (4.3%, p<0.05) were obtained by ES in 36 hr-matured oocytes compared to other treatments (16.5 and 20.5%) in the same age and the same treatment in 44 hr-matured oocytes (15.0%). High blastocyst formation rate (23.6%) was obtained by ES+ES+D in 44 hr-matured oocytes (p<0.05). These results demonstrate that porcine oocyte activation and in vitro development of parthenotes can be affected by interactions between oocyte maturational age and activation condition.
목차
INTRIDUSTION
MATERIALS AND METHODS
In Vitro Maturation of Oocytes
Evaluation of Nuclear Maturation
MPF Kinase Assay
Parthenogenetic Activation
In Vitro Culture
Statistical Analysis
RESULTS
Maturation Pattenrs of Porcine Oocytes
cdc2 Kinase Activity
Development of Parthenotes
DISCUSSION
REFERENCES
저자정보
참고문헌
- 1(2000) Effects of wortmannin on the kinetics of GVBD and the activities of the maturation-promoting factor and mitogen-activated protein kinase during bovine oocyte maturation in vitro,
- 2(1999) Production of goats by somatic cell nuclear transfer,
- 3(2000) Development of reconstituted pig embryos by nuclear transfer of cultured cumulus cells,
- 4(2002) Effect of elevated Ca2+ concentration in fusion/activation medium on the fusion and development of porcine fetal fibroblast nuclear transfer embryos,
- 5(1995) Beneficial effect of oocyte activation prior to and during nuclear transfer in cattle using in vitro matured oocytes 24 hr of age,
- 6(1992) Intracellular Ca2+ response of rabbit oocytes to electrical stimulation,
- 7(1995) Pronuclear formation and intracellular glutathione content of in vitro-matured porcine oocytes following in vitro fertilization and/or electrical activation,
- 8(1991) Response of porcine oocytes to electrical and chemical activation during maturation in vitro,
- 9(2001) Progression of nuclear maturation and p34cdc2 kinase activity in porcine oocytes during in vitro culture in different media,
- 10(2000) Requirement of mitogen-activated protein kinase activation for the meiotic resumption of porcine oocytes,