earticle

영어

Oregonin, a glycoside of diarylheptanoid, is a distinctive compound in Alnus japonica renowned for its anti-inflammatory and antioxidant properties. We investigated aimed in vitro tissue culture conditions at mass-propagation of in vitro plantlets and enhancing Oregonin production. Cultures were initiated in WPM supplemented with plant growth regulators (PGRs) including BA, 2IP, TDZ, zeatin at 1μM, and gibberellin (GA3) at 1μM. Growth parameters such as fresh weight, number of shoots, shoot length, and leaf count were evaluated, and oregonin content was quantified using HPLC. The BA treatment demonstrated superior growth, followed by zeatin, whereas the control and GA3 treatments exhibited inferior performance. Interestingly, despite lower growth, the control group showed the highest Oregonin content. Zeatin treatments generally resulted in higher Oregonin levels. In subsequent experiments comparing various concentrations of BA (1, 5, 10, 15μM), and zeatin (1, 5, 10, 15μM), the 5μM BA treatment showed optimal growth, and among zeatin treatments, 10μM. However, zeatin exhibited superior growth and Oregonin content than BA treatment. Thus, zeatin at 10μM is recommended as the optimal condition for Oregonin production.