earticle

영어

The purpose of this study was to investigate the effect of combining low-intensity treadmill exercise with naringenin treatment on the ex-pression of axonal regrowth-related proteins following sciatic nerve in-jury (SNI). The extracts were evaluated for cytotoxicity and cell viability using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, and the effects of the extracts were analyzed in vitro using pri-mary cultured Schwann cells and dorsal root ganglion neurons (DRGs). In vivo, axonal regrowth-related protein expression levels and neurite outgrowth were assessed through Western blot and immunofluores-cence staining, respectively. The results indicated that neither extract exhibited cytotoxicity. In primary cultured Schwann cells, 10 μM nar-ingin and 10 μM/50 μM naringenin significantly increased growth asso-ciated protein-43 (GAP-43) expression, while in DRGs, both naringin and naringenin treatments resulted in increased neurite length. For in vivo experiment, all animals were divided into the vehicle group, the nar-ingin-treated group post-SNI (Gin), the naringenin-treated group post-SNI (Genin), the naringin and exercise group post-SNI (Gin+Ex), and the naringenin and exercise group post-SNI (Genin+Ex). Naringenin treat-ment after early SNI enhanced GAP-43 expression. Following 14 days of exercise combined with treatment, both GAP-43 and phosphorylated extracellular signal-regulated kinase levels were significantly increased in the Genin and the Genin+Ex groups, whereas phosphorylated-protein kinase B significantly increased only in the Genin+Ex group. Our findings suggest that naringenin, when used in conjunction with low-intensity treadmill exercise, may effectively promote the expression of axonal growth-related proteins following SNI.