earticle

영어

This study aimed to investigate the antibacterial effectiveness of various combinations of Saccharina japonica extract, Centella asiatica extract, and bamboo leaf extract at different concentrations as disinfectants. To achieve this, Saccharina japonica was extracted using distilled water, with 0.01M acetic acid serving as a control. The combination ratio was fixed at 1:1:1, while varying the concentrations of Chamaecyparis obtusa water and ethanol for the experiments. The antibacterial activity was assessed using the disk diffusion method with E. coli and S. aureus, measuring the diameter of the clear zone. The results indicated that after 20 hours, the highest antibacterial activity was found in the 0.01M acetic acid Saccharina japonica extract, with clear zones measuring 7.8 mm for S. aureus and 8.1 mm for E. coli. After 40 hours, this extract showed clear zones of 8.6 mm for both S. aureus and E. coli. These findings demonstrated that the combination of Saccharina japonica distilled water extract, Centella asiatica extract, and bamboo leaf extract in a 1:1:1 ratio, along with Chamaecyparis obtusa water at 82% and ethanol at 15%, exhibited antibacterial activity against both E. coli and S. aureus. This confirms that the natural extracts from SD, CA, and BVL have antibacterial efficacy as disinfectants at the tested concentrations.

중국어

本研究旨在通过实验验证不同浓度的海带提取物􀕵积雪草提取物和竹叶提取物作为杀菌剂的 抗菌功效. 海带使用蒸馏水提取, 对照组使用0.01M醋酸进行提取. 配比固定为1:1:1, 使用不同 浓度的扁柏水和乙醇进行实验. 实验使用大肠杆菌和金黄色葡萄球菌, 通过纸片扩散法测量抑菌 圈直径来确认抗菌活性. 结果显示􎨳20小时后最高抗菌活性出现在0.01M醋酸海带提取物中, 金黄色葡萄球菌为7.8mm, 大肠杆菌为8.1mm. 40小时后, 0.01M醋酸海带提取物中金黄色葡 萄球菌和大肠杆菌均显示8.6mm的抑菌圈. 结果表明, 在大肠杆菌和金黄色葡萄球菌中都显示抗 菌活性的是海带蒸馏水提取物, 积雪草提取物, 竹叶提取物1:1:1配比, 扁柏水82%, 乙醇15% 浓度. 这些结果确认了SD, CA, BVL不同配比浓度作为消毒剂具有抗菌效能的天然提取物.