earticle

영어

Dalbergia sissoo and Dalbergia latifolia are the two most important timber wood species that are indigenous to the Indo-Pak subcontinent producing very high-quality timber. Its wood is used to produce high-quality furniture. Due to overexploitation and dieback disease, their production is seriously hampered and threatens their genetic diversity. Several ecotypes are tolerant to dieback thus offering an opportunity to develop a superior hybrid for the establishment of plantations for hybrid seed production. Hybrid evaluation can only be done by using DNA markers such as SSR markers. Cross-specific amplification of SSR markers is a cost-effective way of producing DNA markers for species lacking genetic information. Here, we report for the first-time successful cross-amplification of SSR markers in Dalbergia latifolia and added new SSR markers in D. sissoo. Cross-species amplification resulted in 13 successful SSR markers in D. latifolia and an addition of 14 markers in D. sissoo of expected sizes. Six SSR markers were further selected randomly to validate the breeding behavior of both species. A diverse DNA profile of seed progenies matched to different pollen donors deviated from the same mother suggested cross-pollination is the most likely mechanism of seed production in D. sissoo and D. latifolia. However, the results must be validated by using a large sample size and through controlled pollinations. SSR markers thus developed will be useful in the conservation and development of superior hybrids for sustainable development and production of commercial populations in Dalbergia sissoo and D. latifolia.