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Background: This study examined the effect of ankle stabilizing exercise, including gluteus exercises and ankle exercises on the pain, function, and balance of patients with chronic ankle instability. Methods: After randomly classifying 15 people per group into the experimental group and control group. The experimental group performed stabilizing exercise including gluteus exercises, the control group performed stabilizing exercise including ankle exercises. Each group performed the exercise three times per week for four weeks. The SPSS 23.0 program was used for statistical analysis. Results: Significant differences in visual analogue scale (VAS), cumberland ankle instability tool (CAIT), star excursion balance test (SEBT) were observed in the experimental group and control group(p<.05). A comparison after intervention between each other groups revealed, a significant difference in the VAS (p<.05), but no significant difference in CAIT and, SEBT (p>.05). Conclusion: These results suggest that stabilizing exercise, including gluteus muscles and ankle exercises are encouraged because of the effects on pain, ankle instability decrease, and Dynamic balance increase in patients with chronic ankle pain