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Background: The most common form of injury in soccer is a hamstring strain. Eccentric bias exercises are crucial for reducing injury risk and improving sprinting performance. The purpose of this study was to compare a 4-week hip-dominant hamstring strengthening exercise program (HDE) with a knee-dominant hamstring strengthening exercise program (KDE) in youth soccer players. Method: The sample (n=31) was randomly divided into two groups: HDE (n=17) and KDE (n=14), with soccer players in HDE performing hip joint-dominant hamstring strengthening exercises and those in KDE performing knee joint-dominant hamstring strengthening exercises. Each intervention was conducted three times per week for four weeks. The outcome measures included the hamstring strength test (isometric strength test, eccentric strength test), knee joint range of motion (90/90 active knee extension; AKE) test, lower limb function test Y-balance test and Agility T-test. Results: Following the intervention, both groups showed significant improvement in isometric and eccentric hamstring strength on both dominant and non-dominant sides (p<.05). The AKE test showed significant improvement in the dominant leg (p<.05) and non-dominant leg only in KDE. The agility test significantly improved in HDE (p<.05). There was a slight improvement in the Y-balance test score; however, the difference was not statistically significant. There were no differences between the two groups in the values before, and after the intervention for all items. Conclusion: The hip-dominant and knee-dominant hamstring strengthening exercise should be coordinated and used based on the environment during hamstring injury prevention training.