earticle

영어

This study aimed to explore the potential application of Salix gracilistyla leaf extract to the skin, evaluating its antioxidant effects and protective properties against oxidative stress using human-derived keratinocytes. The findings revealed that the extract exhibited significant DPPH radical scavenging activity with an IC50 value of 109.29± μg/mL, indicating a dose-dependent increase in scavenging activity. Furthermore, the extract contained notable levels of total polyphenols (203.13±3.25 μg/mL) and flavonoids (154.31±1.16 μg/mL). No cytotoxicity was observed in HaCaT cells at concentrations below 100 μg/mL. Additionally, the extract demonstrated dose-dependent protective effects against hydrogen peroxide-induced oxidative stress. These results imply that Salix gracilistyla leaf extract holds promise for antioxidant skincare applications, potentially mitigating harmful various factors that can damage the skin. Further research in this area is expected to deepen our understanding of its benefits and applications.

중국어

本研究旨在于确认藤柳叶提取物的抗氧化效果及其对皮肤的适用性，本研究利用藤柳叶提取物的抗氧化作用及人源皮肤角质形成细胞，测定了其细胞毒性。研究结果表明，DPPH自由基清除能力IC50值为109.29±μg/mL，清除能力依赖浓度呈现出增加趋势。总多酚含量为203.13±3.25μg/mL，黄酮含量为154.31±1.16μg/mL。HaCaT角质细胞的细胞毒性在浓度为100μg/ml以下时，未呈现出细胞毒性，过氧化氢引起的对氧化压力的保护作用则依赖其浓度呈现出显著的保护效果。基于上述结果，可以推断藤柳叶提取物对皮肤具有抗氧化效果，并能够保护及消除皮肤有害因素。因此，今后需要对此进行持续的研究。