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Purpose: The objective of this study is to identify the differences in muscle coordination patterns during squat exercises based on varying levels of proficiency. Methods: The study included an experienced group (nine participants, age 31.3±1.5 years, height 174.7±1.3 cm, weight 81.4±2.1 kg) and a novice group (eight participants, age 31±1.1 years, height 176.8±2.7 cm, weight 75.1±4.4 kg). Muscle activation levels in the trunk and lower extremities were measured, and muscle synergy analysis was conducted to determine muscle coordination patterns during squats. The extracted muscle synergies were classified using the k-means clustering method. Results: The muscle synergy analysis revealed 3.11±0.33 muscle synergies in the experienced group and 2.75±0.46 in the novice group. The k-means clustering results identified a total of five clusters, with differences in cluster preferences between the experienced and novice groups. Experienced individuals preferred clusters that used large muscles in the lower extremities during squats, while novices favored clusters that engaged smaller muscles in the distal parts of the lower extremities. Conclusion: These findings suggest that experienced individuals use larger muscles to more effectively handle weight during squats, whereas novices rely more on smaller muscles, which may result in lower resistance to weight and a higher risk of injury. The results of this study can serve as foundational data for beginners to learn efficient training methods and develop strategies to prevent injuries.