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Purpose: The purpose of this study was to investigate the effect of the Feldenkrais Method® on the improvement of shoulder joint function in women with desk jobs. Methods: We assessed 14 white-collar women aged 20–30 years. The range of motion of the shoulder joint, cervical mobility, muscle function, and shoulder flexibility were measured. The intervention program used was the Feldenkrais Awareness Through Movement (ATM), which lasted 70 min and included warm-up and cool-down exercises. Results: The changes in shoulder joint mobility after Feldenkrais ATM were significantly improved in terms of abduction (p=0.001), adduction (p=0.005), flexion (p=0.003), extension (p=0.001), internal rotation (p=0.001), and external rotation (p=0.001). The cervical mobility (p=0.022) and muscle function (p=0.001) were also significantly improved. Changes in shoulder flexibility showed significant effects in both the right arm (p=0.003) and left arm dominant postures (p=0.017). Conclusion: The Feldenkrais Method® can increase joint mobility in the shoulder and can be a good way to improve cervical mobility, muscle function, and shoulder flexibility. These effects are thought to be a good way to prevent pain and improve functional impairment for those with shoulder problems.