In Vitro Biological Activities and Relative Chemicals in Different Extracts of Licorice and Fermenting Licorice

Zaijun Geng; Miaomiao Guo; Huirong Zhang; Guanjie Song; Xiaohui Zhang; Xiaoyan Gong; Li Li

원문정보

감초 및 발효감초의 다양한 추출물의 In Vitro 생물학적 활성 및 관련 화학물질

Zaijun Geng, Miaomiao Guo, Huirong Zhang, Guanjie Song, Xiaohui Zhang, Xiaoyan Gong, Li Li

한국피부과학연구원 아시안뷰티화장품학술지 제22권 제2호 통권 제80호 2024.06 pp.185-196 KCI 등재

피인용수 : 0건 (자료제공 : 네이버학술정보)

초록

영어

Purpose: This paper aims to qualitatively and quantitatively analyze the differences between the main chemicals in different licorice and fermented licorice (FL) extracts and their in vitro skin care activities and active ingredients. To evaluate the effect of fermentation on the efficacy of licorice. Methods: The DPPH free radical scavenging activity, mushroom tyrosinase activity, tyrosinase activity of B16F10 cells, and melanin synthesis inhibition activity of different extracts treated by fermentation and different extracts without fermentation treatment were evaluated. The effects of ethyl acetate components (EaF) including glycyrrhizin, isoglycyrrhizin, liquiritoside, isoliquiritigenin and glycyrrhizin ketone A on melanin synthesis were tested in vitro. Further assessment of FL at stromal metalloproteinase-1 (MMP-1) levels in human dermal fibroblasts (HDF). Results: Glycyrrhizin, isoglycyrrhizin, liquiritoside, isoliquiritigenin were improved in FL, and the antioxidant and MMP-1 inhibitory activities of FL controls were significantly increased. Conclusion: After fermentation, the anti-aging effect of licorice was greatly improved, indicating that the fermentation process had a good effect on improving the efficacy of licorice.

한국어

목적: 본 논문은 감초와 발효감초(FL) 추출물의 주요 화학성분과 이들의 in vitro 피부미용 활성 및 유효성분의 차이를 정성적 및 정 량적으로 분석하고자 한다. 또한 감초의 효능에 대한 발효의 영향을 평가한다. 방법: 발효 추출물과 무발효추출물의 DPPH 자유 라 디칼 소거 활성, 버섯 티로시나제 활성, B16F10 세포의 티로시나제 활성 및 멜라닌 합성 억제 활성을 평가하였다. 글리시리진, 이 소글리시리진, 리퀴리토사이드, 이소리퀴리티게닌 및 글리시리진 케톤 A를 포함한 에틸 아세테이트 성분(EaF)이 멜라닌 합성에 미치는 영향을 확인하기 위해 in vitro 시험을 진행하였다. 또한 FL의 인간 진피 섬유아세포(HDF)에서 MMP-1의 발현을 확인하였다. 결과: 글리시리진, 이소글리시리진, 리퀴리토사이드, 이소리퀴리티게닌은 FL에서 개선되었으며, FL의 항산화 및 MMP-1 저해 활 성이 유의하게 증가하였다. 결론: 발효 후 감초의 항노화 효과가 크게 향상되었으며, 이는 발효 과정이 감초의 효능 향상에 좋은 효 과가 있음을 나타냅니다

중국어

目的: 对不同甘草和发酵甘草 (FL)提取物中主要化学物质的差异及其体外护肤活性和活性成分进行定性和定量分析。评价发酵对甘草功效的影响。方法: 评价不同提取物和不同提取物对B16F10细胞的DPPH自由基清除活性、蘑菇酪氨酸酶活性、酪氨酸酶活性和黑色素合成抑制活性。在体外测试了在体外测试了甘草甜素、异甘草甜素、甘草苷、异甘草苷和甘草甜素酮A等乙酸乙酯组分 (EaF)对黑色素合成的影响。进一步评估人真皮成纤维细胞(HDF)中基质金属蛋白酶-1 (MMP-1)水平。结果: 甘草甜素、异甘草甜素、甘草苷、异甘草苷在FL中得到改善，FL的抗氧化和MMP-1抑制活性显著提高。结论: 发酵后甘草抗衰老效果显著提高，说明发酵过程对提高甘草药效有较好的效果。

Abstract
Introduction
Material and Methods
1. Chemicals and regents
2. Extraction method and preparation of fermented licorice
3. Cell culture
4. DPPH radical scavenging activity
5. Mushroom tyrosinase assay
6. Cell viability assay
7. Assay of cellular tyrosinase activity
8. Measurement of melanin content
9. Enzyme-linked immunosorbent assay
10. Total flavonoid content
11. Total polyphenol content
12. Total polysaccharide content
13. Quantitation of the main constituents by HPLC/DAD
14. Data analysis
Results and Discussion
1. DPPH radical scavenging activity
2. Mushroom tyrosinase inhibition activity
3. Evaluation of the cellular effects
4. Total flavonoid, protein, and polysaccharide contents
5. Quantitation of the main constituents of different fractions, FL and NFL
Conclusion
Author's contribution
References

키워드

저자정보

Zaijun Geng 경재군. College of Chemistry and Materials Engineering, Beijing Technology&Business University, Beijing, China; Beijing Key Lab of Plant Resource Research and Development, Beijing Technology and Business University, Beijing, China
Miaomiao Guo 곽묘묘. College of Chemistry and Materials Engineering, Beijing Technology&Business University, Beijing, China; Beijing Key Lab of Plant Resource Research and Development, Beijing Technology and Business University, Beijing, China
Huirong Zhang 장혜영. College of Chemistry and Materials Engineering, Beijing Technology&Business University, Beijing, China; Beijing Key Lab of Plant Resource Research and Development, Beijing Technology and Business University, Beijing, China
Guanjie Song 송관결. College of Chemistry and Materials Engineering, Beijing Technology&Business University, Beijing, China; Beijing Key Lab of Plant Resource Research and Development, Beijing Technology and Business University, Beijing, China
Xiaohui Zhang 장소혜. College of Chemistry and Materials Engineering, Beijing Technology&Business University, Beijing, China; Beijing Key Lab of Plant Resource Research and Development, Beijing Technology and Business University, Beijing, China
Xiaoyan Gong 공효연. College of Chemistry and Materials Engineering, Beijing Technology&Business University, Beijing, China; Beijing Key Lab of Plant Resource Research and Development, Beijing Technology and Business University, Beijing, China
Li Li 리려. College of Chemistry and Materials Engineering, Beijing Technology&Business University, Beijing, China; Beijing Key Lab of Plant Resource Research and Development, Beijing Technology and Business University, Beijing, China

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