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Objectives: This study aimed to identify factors associated with latent tuberculosis(LTB) infection among workers in tuberculosis(TB) hospitals. Methods: Participants were 244 workers at two TB hospitals who responded to structured questionnaires. Results: The prevalence of LTB infection among workers was 25.8%. After controlling for other covariates, workers aged 50 or older had 4.86 times higher risk of LTB infection compared to the group aged 39 or younger. Workers working in hospitals equipped with a partially negative pressure system were 3.82 times more likely to be infected with LTB than those working in hospitals with an entire negative pressure system. The risk of LTB infection was 19.26 times higher for workers in the group with the highest LTB belief score(33.3%) than those in the bottom 33.3%. Conclusions: The results highlight the importance of TB hospitals equipped with a negative pressure system in all wards and multi-faceted efforts to reduce barriers to the prevention of LTB infection among workers.