earticle

영어

In this study, the genetic variations of 36 wild accessions were investigated using SSR markers. Twenty-eight accessions were obtained from the National Institute of Horticultural Science and Technology, three accessions from Gangwon Forest Science Institute, and two accessions from Gangwon Agricultural Research Institute. Sixty SSR primer sets were designed from genomic information available publically and tested for PCR amplification using pooled DNA from the accessions. Twenty-five primer sets exhibited polymorphism and were used for further analysis. The average number of alleles was 4.64 per primer, and the observed and the expected average heterozygosity were 0.435 and 0.540, respectively. The average polymorphism information content was 0.568. UPGMA cluster analysis showed that 36 accessions were classified into five groups. PCoA analysis showed similar patterns with the UPGMA result. Nevertheless, a limited number of SSR markers were used for genetic relationship analysis, our results would provide valuable information to facilitate the management of selected wild pears and to develop new cultivars in the future.