earticle

영어

Multi-layered cellulose nanocrystal (CNC) structure with hyaluronic acid (HA) and doxorubicin hydrochloride (DOX) was fabricated for tumor-targeted drug delivery. DOX was coated to the outer surface of rod-shaped CNC via an electrostatic interaction. Then, DOX@CNC composites were wrapped with HA for CD44 receptor-mediated endocytosis in cancer cells. Anionic charged HA was bound to DOX@CNC for CD44 receptor-positive tumor-targeted drug delivery. HA@DOX@CNC with 327 nm length, 12 nm width, -38 mV zeta potential, and 3% drug content was successfully prepared. Designed HA@DOX@CNC composite exhibited higher uptake and antiproliferation efficiencies in lung adenocarcinoma (A549 cells). Cy5.5 (which is one of near-infrared fluorescence dyes)-conjugated HA-covered DOX@CNC was injected intravenously to A549 tumor-implanted mouse model and the biodistribution profile was monitored by optical imaging. HA@DOX@CNC group displayed higher tumor accumulation efficiency following intravenous injection. Also, it did not induce any serious systemic toxicities. It can be concluded that HA@DOX@CNC can be used efficiently and safely for systemic cancer therapy.