원문정보
초록
영어
Generally, bacteriocins are known as antimicrobial proteins produced from lactic acid bacteria. Bacteriocins induce the cell death of bacteria through several mechanisms such as forming holes in the cell membrane. These bacteriocins are considered as substitutes for conventional antibiotics causing antibiotic resistance problems. We isolated a lactic acid bacteria showing antimicrobial activity, and identified this strain as Lactobacillus plantarum BG0001 through 16S RNA sequencing. After L. plantarum BG0001 was cultured in MRS medium for 24 hours, culture broth was separated by centrifugation and concentrated to 20-fold by freeze drying. Using this concentrated culture broth, disk diffusion test and minimal inhibition concentration (MIC) test were performed with Bacillus cereus for verifying antimicrobial activity. According to disk diffusion test, the sizes of clear zone were gradually and proportionally increased as increase as the concentration of concentrated culture broth. As a result of MIC test, the growth of B. cereus was highly inhibited from the concentration of 12.79 mg/mL bacteriocin produced by L. plantarum BG0001.