원문정보
초록
영어
The pine cones of Pinus pumila were extracted by ethanol. From the resulted residues, the polysaccharides of P. pumila cones were obtained by hot water extraction, spray drying and alcohol precipitation processes. The content of the polysaccharides was determined by phenol sulfuric acid method. The analysis of monosaccharide composition of the polysaccharide conducted by gas chromatography revealed that it was a heteropolysaccharide and consisted of L-arabinose, D-mannose, D-glucose and D-galactose in a molar ratio of 2.33:1.00:2.20:1.94, which indicated typical IR characteristics of polysaccharides. In addition, the antioxidant activity of polysaccharides was evaluated by measuring DPPH free radical and ABTS free radical scavenging capacity, as well as reducing capacity, and then the immunoregulatory activity of polysaccharides against RAW264.7 macrophages was evaluated by in vitro experiments. The results showed that pine cones polysaccharides of P. pumila exhibited significant scavenging effects on DPPH radicals, ABTS+ radicals and strong reducing power in a concentration-dependent manner. In addition, polysaccharides of P. pumila cones could stimulate RAW264.7 macrophages to produce a large amount of NO, but had no effect on cell proliferation. Overall, polysaccharides from Pinus pumila cones may have potential applications in the medical and food industries.