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논문검색

Molecular identification and sex determination for unidentified specimens of artiodactyl mammals collected in Mt. Yongmasan, Seoul, South Korea

초록

영어

Recently several observations have been reported on the mass-media about habitation of long-tailed goral (Naemorhedus caudatus) in eastern borderline (Mt. Yongmasan) of the metropolitan city, Seoul. Non-invasive and unidentified specimens (hairs and pellets) were collected and we tested molecular analyses such as species identification, molecular sexing and individual counting. Nucleotide sequences of mitochondrial DNA cytochrome B and cytochrome oxidase I genes showed that those samples were water deer (Hydropotes inermis) and long-tailed goral. In addition from the results of molecular sexing we found a male and a female long-tailed goral DNA samples using male-specific sex-related region Y gene and sexually heterogenous zinc finger -X and -Y genes, respectively. The genotypes of microsatellite markers differentiated the DNA samples into a water deer and two individuals of long-tailed goral. Combining whole molecular information, we finalized that those specimens might be from at leat a water deer and two long-tailed goral. From the camera trapping (CT) survey, we also took some photos of male goral supposed as the same individual from several locations. However, we could not caught the female one at that time by CT. Although we have no direct evidence on habitation of female goral until now, our molecular assay results indicated that both male and female goral inhabit Mt. Yongmasan, Seoul. Anyway, this is the new and first record about habitation of long-tailed goral nearby the metropolitan city Seoul where might be the farthest away to the west from the main habitat (far eastern mountainous ranges of South Korea). Our findings concluded that further survey should be carry out to estimate the number of individuals by molecular test and camera trapping together, and it is necessary to establish a conservation program for the long-tailed goral in Mt. Yongmasan, Seoul.

저자정보

  • Ju-Young Moon Species Restoration Technology Institute, Korea National Park Service
  • Chea-Un Cho Species Restoration Technology Institute, Korea National Park Service
  • Tae-Wook Kim Species Restoration Technology Institute, Korea National Park Service
  • Jang-ick Son Species Restoration Technology Institute, Korea National Park Service, Gurye 57616 & Inje 224606, Republic of Korea
  • Gyu-cheol Kim Species Restoration Technology Institute, Korea National Park Service, Gurye 57616 & Inje 224606, Republic of Korea
  • Yong-hak Lee Species Restoration Technology Institute, Korea National Park Service
  • Seung-Jun Jeong Species Restoration Technology Institute, Korea National Park Service
  • Dong-ju Song Species Restoration Technology Institute, Korea National Park Service
  • Sang-Hyun Han Species Restoration Technology Institute, Korea National Park Service

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