원문정보
초록
영어
Understanding the genetic information and diversity assessment are important for the restoration strategies to manage endangered species. Since the beginning of fox restoration in Korea, foxes of the same subspecies have been introduced for restoration for five years, but there has been no evaluation that the introduced species are genetically healthy. The aim of this study was to estimate the genetic diversity of the introduced 11 Red fox individuals. single nucleotide polymorphism (SNPs) using RNA-sequencing discovered for analyzing genetic diversity. As there are no genomic reference resources of Vulpes vulpes, Transcriptome database were made from 14 individuals as a reference data. Using this reference data, SNPs were detected and nucleotide diversity (π) was calculated. The mean of π was 2.287*10-4bp-1, and median was 1.967*10-4bp-1. This calculated result of π was compared to other wild species’, including the species in the IUCN Red List of threatened species. The genetic diversity of 11 introduced Red foxes had relatively lower diversity. Scattered plot based on PCA of 11 individuals showed that the low degree of variance, this indicate that genetic diversity among individuals was very low.