원문정보
초록
영어
Catechin belonging to the tannins are found in various kinds of tea, and are mainly contained in green tea. It has a strong reducing effect and have antioxidant, antiviral, antibacterial effect as well. In addition, derivatives produced from catechin degradation are known as high value-added substances. Previously, we isolated two bacteria capable of degrading catechin from soil. These two bacteria were identified as Enterobacter sp. Several genes were cloned to identify catechin degrading enzymes from two isolated bacteria. Using cloned genes, catechin degrading enzyme overexpression systems were constructed with pET-21a vector. As results of overexpression using a recombinant E. coli, a soluble from enzyme was overexpressed. This enzyme exhibited catechin degradation activity after purification using 6His tag.