원문정보
초록
영어
Due to increased environmental concerns and needs for energy security, biofuel productions have become attractive alternatives to the fossil fuels. The production of biofuels from edible starch were suffer from ethical problems of using food resources. Biofuels production using non-edible cellulosic biomass has been attempted to solve those problems. Xylose, one of the main components of cellulosic biomass, can be converted to xylitol which has been known as a valuable synthetic building block. In this study, K. marxianus KCTC17555ΔURA3 strain was engineered by multi-copy random integration of the mutant KmXYL1 (mKmXYL1) gene from K. marxianus 36907-FMEL1. When fermentation experiments were performed using xylose as a sole carbon source at 40°C, K. marxianus 17555-JBP2 produced 53.17 g·L-1 of xylitol with 0.73 g·g-1 yield and 0.55 g·L-1·h-1 productivity. In conclusion, K. marxianus 17555-JBP2 showed 190% and 300% improvement of xylose consumption rate and xylitol production rates, respectively.