원문정보
초록
영어
Chloroplast genome sequences among wild and cultivated Prunus yedoensis accessions were compared to develop individual specific DNA markers. Prunus yedoensis is one of most popular ornamental tree species planting in roadside and parks in Korea. Also, there are many natural population in Jeju-Island that the wild morphological characteristics are not distinguishable to cultivate. We compared the chloroplast genome sequence and made up consensus cp genome sequence using the 6 individuals, in which two cultivates and 4 wild individuals are collected. The 4 complete cp genome sequence are generated by de novo assembly of the sequencing data generated by Illumina high-throughput sequencing platform and the other two cp genome sequence were obtained from Genebank. The consensus sequence was aligned as 158,149 bp with containing 86,210 bp of LSC, 19,153bp of SSC, and each 26,393 bp of IRA and IRB. A total AT ratio is 63.28% in consensus sequences. Although these are identified same species with the morphological characteristics, we found highly variable sequence in cp genome. A total of 168 SNPs and 531 INDELs were identified and these take 0.106% and 0.336% respectively. One individual was identified highly different cp genome from the others by neighbor-joining tree analysis. This would be good resource to study of genetic diversity in Prunus yedoensis. We identified SSRs from the cp genomes that discriminate the accessions and possibly use for the cultivar specific markers in the further investigation.