earticle

영어

Cudrania tricuspidata (Moraceae) has long been used as a traditional medicine and food material. It is widely distributed in East Asia, mainly in the southern part of Korea. In this study we evaluated the previously developed UPLC analytical method to quantify four major isoflavonoids components including 6,8-diprenylgenistein, 6,8-diprenylorobol, alpinumisoflavone, and 4´-O-methylalpinumisoflavone in C. tricuspidata fruits. Based on this analytical method, non-targeted metabolite profiling of immature fruits of C. tricuspidata at four different regions in Korea was performed using ultraperformance liquid chromatography–quadrupole time-of-flight mass spectrometry (UPLC-QTOF MS) technique. Further, various multivariate analyses were applied to find the biomarkers according to the regional variances. The UPLC-QTOF MS-based analytical method as well as multivariate analyses suggested more reliable results for precise authentication and quality assurance of C. tricuspidata fruits.