earticle

영어

Venom of spiders is composed of various peptides, which have distinct physiological characteristics. We collected venom derived from domestic indigenous spiders including Argiope bruennichi and Pardosa astrigera, and proceeded evaluation for antimicrobial and anticancer activities. Growth inhibitory effects of spider venom against gram-positive (B. cereus and S. aureus), gram-negative (E. carotovora and E. coli) bacteria, and fungi (F. oxysporum) was determined, respectively. Analysis of cell membrane permeability showed that the venom from both spiders possess significant antimicrobial activity via increasing bacterial and fungal cell permeability. Cytotoxic effects of the spider venom were performed on normal cell line (hAD-MSCs) and cancer cell lines (HepG2, AGS, HeLa, and H460 cells). We found anti-proliferative activity of venoms against cancer cells, while the up-regulation of apoptosis related genes appeared to be correlated with such cytotoxic effects against cancer cell lines. De novo peptide sequencing was conducted, and partial sequences of venom peptides were verified. We found that the identified peptides are novel, but share homology with toxin related peptides reported in Araneae database. We selected domestic indigenous spider, A. bruennichi, and performed RNA sequencing to identify the expression of the venom specific genes. We finally identified five novel toxin-like peptides, predicted by utilizing the homology and its structural characterization analysis of acquired genes. These results imply the feasibility in developing antimicrobial and anticancer agents by utilizing novel peptide derived from spider venoms.